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Re: [HAPS-L] "I'm going to pick a fight" - Braveheart



Yes, this is the way I've always taken it. The 'first' messenger is the diffusible extracellular messenger, the second messenger is the diffusible intracellular messenger. Obviously, the terminology is becoming more and more confusing as we learn more details about these signal transduction pathways. If we want to break it down further, when the receptor binds a hormone, if the binding activates a catalytic subunit within the receptor, is that subunit the second messenger?

Perhaps the term 'second messenger' has outlived its usefulness. Maybe it would be best to just use the terms 'extracellular' and 'intracellular' messengers, and the proteins that mediate the signals from outside to inside are simply membrane signal transduction proteins.

I'm just thinking off the top of my head. I always prefer naming conventions that are as simple and logical as possible, but my students need to know the terminology as it's used in the rest of the world, so I can't come up with my own system.

At 04:18 PM 2/8/2006 -0500, you wrote:
I think the rationale is historical, and I sometimes explain it to students this way. In the 1950's, hormones were thought of as THE chemical messengers in the body, and then Sutherland & Rall's experiment showed that some other "second messenger" takes over inside of the cell. http://www.beyonddiscovery.org/content/view.page.asp?I=363 http://nobelprize.org/medicine/laureates/1971/press.html

The term "second messenger" was eventually used for two things: the messenger molecule itself, cAMP, as well as the general concept, the second messenger system. So when additional pathways were discovered, they were also considered examples of "the second messenger system", even when 3 or 4 molecules were involved in the pathway, and the term "second messenger" was sometimes used to mean "intracellular messenger", without regard to what number it was in the pathway. I think it's in this sense that some textbooks refer to IP3, DAG, and Ca++ as all being "second messengers". Adding to the confusion, "second messenger" is usually used to indicate a diffusible molecule, so DAG doesn't fit the bill, even though it resembles IP3 in its placement in the IP3/DAG pathway. And Ca++ may act as second messenger in one pathway and as third messenger in another.

I find Boron and Boulpaep's Medical Physiology to be the best in resolving
these questions of a molecular nature.  Does anyone have a copy of that?

What I tell students is that the terminology is in flux, as it always is at a time when new discoveries are being made. (We had a similar discussion a while back... I think it was around the question of how many organ systems or how many tissue types there are.) I tell students to understand the general concept and the particular pathways, but not worry about whether to call a particular molecule a second or third messenger.


Judy Gibber



On Wed, 8 Feb 2006, Ford, Dayton wrote:

Okay. Would someone please explain to me the rationale behind referring to
calcium as the second messenger in the IP3/DAG second messenger system,
rather than IP3 and DAG. In the cAMP system, cAMP is the molecule made that
then activates a cascade of events and is thus termed the second messenger.
In almost all of the texts that I have seen (including Guyton's 11th ed.)
the second messenger is said to be "Calcium". The only text that seems to
get it right is Berne, Levy, Koeppen, and Stanton's 5th ed. Wherin it is
stated that there are THREE systems. The cAMP system (with cAMP as the
second messenger), the Calcium-Calmodulin system (with Calcium as the second
messenger), and the Membrane Phospolipid system in which IP3 and DAG are the
second messengers. Other second messengers are listed (e.g. cGMP, NO, etc.),
but in an Intro to Physiology course we tend to focus only on the two main
pathways (cAMP, IP3/DAG). I have always argued that the second messengers in
the IP3/DAG system are IP3 and DAG. These are the molecules synthesized by
the enzyme activated by the G-protein. I thus tell my students to ignore
what the text says (Fox in my case) and refer to IP3 and DAG as the second
messengers. It becomes very confusing to my students when they see the cAMP
path in their textbook as:



Receptor --> G-Protein --> Enzyme --> Second Messenger --> Cellular Cascade



While the IP3/DAG system is thus:



Receptor --> G-Protein --> Enzyme --> Intermediate Molecule?? --> Cellular
Effect --> Second Messenger --> Cellular Cascade.



Seems to me that the only textbook that has attempted to clear up this
confusion is the Berne book. Anyone want to explain the rationale behind
this confusing bit of nomenclature??



Dayton J. Ford, Ph.D.

Associate Professor of Biological Sciences

St. Louis College of Pharmacy

4588 Parkview Place

St. Louis, MO  63110

dford@xxxxxxxxxx

314-446-8463 voice

314-446-8460 FAX



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