RE: [HAPS-L] Cell labs

["Carl Shuster" <CShuster@xxxxxxxxxxxxxxx>]

Hi Trevor,

Any chance of talking you into recording this video demo next time you do it?  I'd sure like to see a copy.

Carl S

Carl Shuster
Biology
Madison Area Technical College
(608) 246-6203
CShuster@xxxxxxxxxxxxxxx

>>> "Trevor Day" <taday@xxxxxxxxxxx> 02/20/07 1:40 PM >>>
To add to Bob's RBC suggestion: if you have some RBCs in an isotonic
solution on a slide with a cover slip, use a microscope with a video
camera in one eyepiece and show the whole class on a TV. You can
demonstrate Brownian motion this way as well, as the light heats them up a
bit and you can see them jiggling around. Put a small piece of paper towel
on one side of the slide and add a hypertonic solution to the opposite
side. The paper towel will facilitate movement of fluid, and you'll see
the RBCs crenate before your eyes. Now try washing distilled water
(hypotonic) across the slide...with practice, you can watch them expand
and then 'disappear' (lyse)...with good resolution, you can sometimes see
membrane ghosts too! Cheap and easy group demo to get the point across,
especially if you ask them to make predictions along the way. We used
sheep blood.
God luck.
Trevor


On Tue, February 20, 2007 9:40 am, Rawding, Robert S said:
> Peggy,
>
> The PhysioEx CD bundled with Marieb's and Ric Martini's books has a nice
> simulation on cell membrane function that covers diffusion - passive and
> facilitated, osmosis and osmotic pressure, and active transport.
>
> The lab would require some PC's, though.   There are also some nice labs
> around in which peroxidases are used to illustrate enzyme function -
> they require a Spec20 spectrophotometer.
>
> In both cases we work with the cell models we have available and couple
> that with a short video (whose name eludes me right now).
>
> There is another option too- freshwater 'critters' that are easy to
> maintain in the lab and are fascinating to observe - use amoebas
> (amoebae?) for sol-gel transformations, paramecia, for contractile
> vacuole formation and cilia, Stentor, also for cilia, and maybe a few
> more, some with flagellae.
>
> If someone (you or a student) 'donates' some RBC's, you have a nice
> exercise by preparing isotonic, hypotonic, and hypertonic solutions in
> which to suspend the cells - prepare solutions of each and place a drop
> on a microscope slide.   Then add a tiny drop of red cells.
>
> My 2 cents worth.
>
> Bob
>
>
>
> -----Original Message-----
> From: HAPS-L-owner@xxxxxxxxxxx [mailto:HAPS-L-owner@xxxxxxxxxxx] On
> Behalf Of Peggy LePage
> Sent: Monday, February 19, 2007 4:19 PM
> To: HAPS-L@xxxxxxxxxxx 
> Subject: [HAPS-L] Cell labs
>
> Does anyone have any interesting cell labs...we are doing
> osmolarity/tonicity but need another lab to support their understanding
> of a functional cell.  It seems they know the basics regarding the
> organelles and have a handle on DNA/RNA, protein synthesis but do not
> seem to relate to "how cells function" so the progression to histology
> and myocyte/neuron physiology can seem pretty overwhelming.
> Thanks,
> Peggy
>
>
> ----
> Peggy LePage
> Professor of Biological Sciences
> North Hennepin Community College
> PLEPAGE@xxxxxxxx     peggy.lepage@xxxxxxxxx 
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--
Trevor Day
PhD Candidate
Physiology and Biophysics
Faculty of Medicine
University of Calgary
taday@xxxxxxxxxxx 
(403)220-4528

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