RE: [HAPS-L] Cell labs

["David Evans" <devans@xxxxxxx>]

Get yer RBC's from a livestock supplier. Citrated works best for us. 

-----Original Message-----
From: HAPS-L-owner@xxxxxxxxxxx [mailto:HAPS-L-owner@xxxxxxxxxxx] On
Behalf Of Trevor Day
Sent: Tuesday, February 20, 2007 2:55 PM
To: HAPS-L@xxxxxxxxxxx
Subject: RE: [HAPS-L] Cell labs

...uh...make that 'good' luck. Spell check didn't catch that one...
T

On Tue, February 20, 2007 12:40 pm, Trevor Day said:
> To add to Bob's RBC suggestion: if you have some RBCs in an isotonic 
> solution on a slide with a cover slip, use a microscope with a video 
> camera in one eyepiece and show the whole class on a TV. You can 
> demonstrate Brownian motion this way as well, as the light heats them 
> up a bit and you can see them jiggling around. Put a small piece of 
> paper towel on one side of the slide and add a hypertonic solution to 
> the opposite side. The paper towel will facilitate movement of fluid, 
> and you'll see the RBCs crenate before your eyes. Now try washing 
> distilled water
> (hypotonic) across the slide...with practice, you can watch them 
> expand and then 'disappear' (lyse)...with good resolution, you can 
> sometimes see membrane ghosts too! Cheap and easy group demo to get 
> the point across, especially if you ask them to make predictions along

> the way. We used sheep blood.
> God luck.
> Trevor
>
>
> On Tue, February 20, 2007 9:40 am, Rawding, Robert S said:
>> Peggy,
>>
>> The PhysioEx CD bundled with Marieb's and Ric Martini's books has a 
>> nice simulation on cell membrane function that covers diffusion - 
>> passive and facilitated, osmosis and osmotic pressure, and active 
>> transport.
>>
>> The lab would require some PC's, though.   There are also some nice
>> labs
>> around in which peroxidases are used to illustrate enzyme function - 
>> they require a Spec20 spectrophotometer.
>>
>> In both cases we work with the cell models we have available and 
>> couple that with a short video (whose name eludes me right now).
>>
>> There is another option too- freshwater 'critters' that are easy to 
>> maintain in the lab and are fascinating to observe - use amoebas
>> (amoebae?) for sol-gel transformations, paramecia, for contractile 
>> vacuole formation and cilia, Stentor, also for cilia, and maybe a few

>> more, some with flagellae.
>>
>> If someone (you or a student) 'donates' some RBC's, you have a nice 
>> exercise by preparing isotonic, hypotonic, and hypertonic solutions 
>> in which to suspend the cells - prepare solutions of each and place a
drop
>> on a microscope slide.   Then add a tiny drop of red cells.
>>
>> My 2 cents worth.
>>
>> Bob
>>
>>
>>
>> -----Original Message-----
>> From: HAPS-L-owner@xxxxxxxxxxx [mailto:HAPS-L-owner@xxxxxxxxxxx] On 
>> Behalf Of Peggy LePage
>> Sent: Monday, February 19, 2007 4:19 PM
>> To: HAPS-L@xxxxxxxxxxx
>> Subject: [HAPS-L] Cell labs
>>
>> Does anyone have any interesting cell labs...we are doing 
>> osmolarity/tonicity but need another lab to support their 
>> understanding of a functional cell.  It seems they know the basics 
>> regarding the organelles and have a handle on DNA/RNA, protein 
>> synthesis but do not seem to relate to "how cells function" so the 
>> progression to histology and myocyte/neuron physiology can seem
pretty overwhelming.
>> Thanks,
>> Peggy
>>
>>
>> ----
>> Peggy LePage
>> Professor of Biological Sciences
>> North Hennepin Community College
>> PLEPAGE@xxxxxxxx     peggy.lepage@xxxxxxxxx
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>
>
> --
> Trevor Day
> PhD Candidate
> Physiology and Biophysics
> Faculty of Medicine
> University of Calgary
> taday@xxxxxxxxxxx
> (403)220-4528
>
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--
Trevor Day
PhD Candidate
Physiology and Biophysics
Faculty of Medicine
University of Calgary
taday@xxxxxxxxxxx
(403)220-4528

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