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Physiological Psychology |
DAY COURSE |
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Roger N. Morrissette, PhD |
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Histology
Objectives:
By the end of this laboratory you should be able to do the following:
· Familiarize yourself with the parts of a microscope
· Identify and sketch the different types of epithelial tissue.
· Identify and sketch the different types of connective tissue.
· Identify and sketch the different types of muscle tissue.
· Identify and sketch the different types of nervous tissue.
· Identify and sketch the different types of tissue from endocrine glands.
A tissue is a group of cells that usually has a common structure, function, and embryonic origin. Histology is the term used to define the study of tissues. Today you will all act as histologists and investigate the four types of animal tissue (Epithelial, Connective, Muscle, Nervous). Many slides representing all four tissues will be available to you. You will also look at some organ tissue (a mix of the four tissues) from the endocrine or hormone system. Your job will be to create a histology notebook. This notebook will contain sketches of each tissue and notes about its characteristics. I will leave the characterization up to you. Examples of characteristics to note are color, shape, and border differences. Many slides will contain multiple tissue types although each slide is typically trying to emphasize just one. Please work in groups of three, keep one histology notebook, and take turns sketching and adding notes. In order to get full credit for the laboratory your group will be given 2 mysterious slides at the end of the lab and your task will be to identify each tissue. You may work with your laboratory partners and may use your histology notebooks to identify the tissue on the quiz. It is therefore imperative that you pay close attention to your histological slides, make good sketches, and identify as many characteristics as you can. Good luck.
Before you get started here is an overview of the four types of animal tissue and how they are related to each other:
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Four Types of Tissue · Epithelial o lines the lumen of an organ o secretion/absorption · Connective o offers support and strength · Muscle o movement · Nervous o innervates muscle |
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PARTS OF THE MICROSCOPE
Please familiarize yourself with the parts of the microscope shown in the figure above. For this laboratory, I have preset all of the slides on the microscopes. You will travel from station to station and draw your rendition of the slide in your laboratory notebook. There are only two adjustments to the microscope that you may have to make. The first is with the "eyepiece". You may adjust the eyepiece distance from side to side to match the distance between your own two eyes. If you do this correctly, you will see a clear image with both of your eyes open. The second adjustment that you may have to make is the "fine adjustment" knob. This can be rotated forward or back to focus the image for your particular eye strength. Please do not touch any other aspect of the microscopes and call me over if you are having any trouble seeing an image. Good luck.
Here are the tissues you will need to sketch today:
EPITHELIAL TISSUE
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Simple Columnar Epithelial Tissue |
Stratified Squamous Epithelial Tissue |
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CONNECTIVE TISSUE
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Elastic Connective Tissue |
Bone Connective Tissue |
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MUSCLE TISSUE
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Cardiac Muscle Tissue |
Skeletal Muscle Tissue |
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NERVOUS TISSUE
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Artery, Vein, and Nerve Tissue |
Paccinian Corpuscle Nervous Tissue |
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Spinal Cord Nervous Tissue |
Motor Nervous Tissue |
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Cerebellum Nervous Tissue |
Cerebral Cortex Nervous Tissue |
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ENDOCRINE TISSUE
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Thyroid and Parathyroid Endocrine Tissue |
Testes Endocrine Tissue |
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